Core and Elective Courses

BT 6303 - Proteomics: Applications and Techniques [2 credits]
Overview of contents

Introduction to proteomics, Transition from genomics to proteomics - prospects and challenges, Protein purification and separation technologies, Two-dimensional gel electrophoresis (2-DE), Difference in-gel electrophoresis (DIGE), Fundamentals of mass spectrometry, Soft ionization methods [Matrix assisted laser desorption/ionization-Time of Flight (MALDI-TOF), and Electro-spray ionization (ESI)], Tandem Liquid Chromatography-Mass spectrometry (LC-MS/MS), Next-generation ultra-high resolution mass-spectrometry, Label-based (SILAC, iTRAQ, and TMT) and label-free (LFQ) quantitative proteomics, Applications of proteomics-studying disease pathogenesis, host-pathogen interactions, biomarker discovery, elucidating the mechanism of drug actions, analysis of biomolecular interactions, analysis of post-translational modifications (phosphorylation and glycosylation), and study of biological rhythms and sleep.

Practical component

Mass spectrometry data visualization, database search, analysis of quantitative proteomics data (MASCOT and MaxQuant), interpretation of MaxQuant analysis output files, and statistical analysis of MS-MS data.

BT 6050 - Circadian Clocks: Mechanisms and Functions [2 credits]
Overview of contents

Introduction to biological oscillators, A brief history of circadian timekeeping, Circadian clocks – the rhythms of life, Clock circuits in different organisms, Adaptive significance of rhythms, Molecular mechanisms of transcriptional and non-transcriptional circadian oscillations, Multidimensional regulation of the circadian rhythms, Master and peripheral clocks in mammalian systems, Metabolic oscillations, Timeseries analysis of rhythms - concepts and methods, Physiological functions and importance of time-keeping machinery, Circadian clocks and sleep, Circadian clocks in health and diseases, Human circadian disorders, Clock-infection biology, Chrononutrition- effects of diet on circadian rhythmicity, Chronomedicine, Modulation of daily rhythms for therapeutic benefit.

BT 5050 - Medical Microbiology and Infectious Diseases [2 credits]
Overview of contents

Introduction to Medical Microbiology; Observing Microorganisms through a Microscope; Morphology and nature of microorganisms; Classification, Identification, and Diversity of Bacteria; Bacterial Growth, Physiology and Death; Microbial Metabolism; Sterilization and Disinfection; Antimicrobial Agents; Human Microbiome in Health and Disease; Pathogenicity of Microorganisms; Basic Principles of Adaptive Immunity and Immunization; Interaction Between Microbes and Hosts; Human Diseases Caused by Bacteria; Human Diseases Caused by Viruses and Prions; Human Diseases Caused by Fungi and Protists; Microbial Diseases of the Skin and Eyes, Microbial Diseases of the Nervous System, Microbial Diseases of the Cardiovascular and lymphatic Systems; Microbial Diseases of the Respiratory System; Microbial Diseases of the Oral and Gastrointestinal Systems; Microbial Diseases of the Urinary and Reproductive Systems; Coronavirus disease 2019 (COVID-19); Infection Control and Epidemiology; Systems Level Approaches to Study Infections

BT 2040 - Microbiology [2 credits]
Overview of contents

Evolution of microorganisms and microbiology; Cell structure and organization - prokaryotic and eukaryotic cells; Microbial taxonomy and diversity; Staining and microscopy; Microbial growth; Bacterial culture methods; Microbial metabolism; Control of microorganisms - physical and chemical methods for control of microorganisms, antimicrobial chemotherapy, resistance to antibiotics; Microorganisms in the environment – microbiology of soil, air, freshwater, and seawater; Biogeochemical cycles -carbon cycle, nitrogen cycle, sulfur cycle; Medical microbiology and virology- microbial diseases, detection, and control; Industrial and food microbiology